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a498 cells  (ATCC)


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    ATCC a498 cells
    A498 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a498+cells/A-498/pm42278453-143-5-10
    Average 97 stars, based on 1667 article reviews
    a498 cells - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancer.
    Article Snippet: .. Cultured human embryonic kidney (HEK293) (ATCC, Cat# CRL-1573; RRID:CVCL_0045), (Human, unknown) and HK-2 (ATCC, Cat# CRL-2190; RRID:CVCL_0302), (Human, Male) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich), 786-O cells (ATCC Cat# CRL-1932; RRID:CVCL_1051), (Human, Male) OS-RC-2 (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_1626) cells (Human, Male), and TUHR14TKB (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_5953) cells (Human, Male) in Roswell Park Memorial Institute (RPMI)1640 Medium (Sigma-Aldrich), A498 cells (ATCC Cat# CRL-44; RRID:CVCL_1056), (Human, Female) in Minimum Essential Medium (MEM, SigmaAldrich), Caki-1 (ATCC Cat# HTB-46; RRID:CVCL_0234), (Human, Male) and Caki-2 cells (ATCC Cat# HTB-47; RRID:CVCL_0235), (Human, Male) in McCoy’s 5A Medium (Sigma-Aldrich) all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich). .. Cultured human embryonic kidney (HEK293) (ATCC, Cat# CRL-1573; RRID:CVCL_0045), (Human, unknown) and HK-2 (ATCC, Cat# CRL-2190; RRID:CVCL_0302), (Human, Male) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich), 786-O cells (ATCC Cat# CRL-1932; RRID:CVCL_1051), (Human, Male) OS-RC-2 (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_1626) cells (Human, Male), and TUHR14TKB (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_5953) cells (Human, Male) in Roswell Park Memorial Institute (RPMI)1640 Medium (Sigma-Aldrich), A498 cells (ATCC Cat# CRL-44; RRID:CVCL_1056), (Human, Female) in Minimum Essential Medium (MEM, SigmaAldrich), Caki-1 (ATCC Cat# HTB-46; RRID:CVCL_0234), (Human, Male) and Caki-2 cells (ATCC Cat# HTB-47; RRID:CVCL_0235), (Human, Male) in McCoy’s 5A Medium (Sigma-Aldrich) all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich).

    Article Title: Sialidase-Mediated Desialylation Regulating EGFR Phosphorylation and Signal Flux
    Article Snippet: .. The A498 cells were purchased from ATCC (HTB-44) and cultured under 37°C, 5% CO2 in minimum essential medium (MEM, Gibco, 11095080) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin (Pen/Strep). .. For sialidase treatment, 50 μl of α2–3,6,8,9-neuraminidase A (20,000 U/mL, New England BioLabs) from Arthrobacter ureafaciens was added to the empty cell culture media (without FBS) and the treatment were performed 2h following previously established protocols .

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: Caki-1 cells were cultured in McCoy medium (ATCC 30-2007) + 10% heat-inactivated FCS (Eurobio Scientific CVFSVF00-01) + 1× penicillin/streptomycin (Gibco 15140-122). .. A498 cells and BJ fibroblasts were cultured in Eagle Minimum Essential Medium (ATCC 30-2003) + 10% heat-inactivated FCS + 1× penicillin/streptomycin + 1× HEPES (Gibco 15630-080). .. All cell lines were cultured in a humidified atmosphere of 5% CO2 at 37°C and routinely tested for Mycoplasma (Lonza LT07-318).

    Article Title: Dose-Dependent Inhibition of Protein Glycosylation Reveals Crosstalk between Glycosylation and Phosphorylation in EGF Signaling Pathways.
    Article Snippet: Protein glycosylation and phosphorylation play critical roles in regulating diverse cellular processes, yet their interplay remains underexplored.. This study employed dosedependent glycosylation inhibition to investigate the function of glycosylation, especially in phosphorylation in epidermal growth factor (EGF) signaling.. Using quantitative proteomics, we analyzed global protein expression, glycopeptides, and phosphopeptides in EGF-stimulated and unstimulated conditions.

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton
    Article Snippet: Caki-1 cells were cultured in McCoy medium (ATCC 30-2007) + 10% heat-inactivated FCS (Eurobio Scientific CVFSVF00-01) + 1× penicillin/streptomycin (Gibco 15140-122). .. A498 cells and BJ fibroblasts were cultured in Eagle Minimum Essential Medium (ATCC 30-2003) + 10% heat-inactivated FCS + 1× penicillin/streptomycin + 1× HEPES (Gibco 15630-080). .. All cell lines were cultured in a humidified atmosphere of 5% CO 2 at 37 °C and routinely tested for Mycoplasma (Lonza LT07-318).

    Article Title: Synergistic and Selective Antiproliferative Effects of Cafestol and a Hyaluronic Acid-Epigallocatechin Gallate Conjugate in Human Renal Cancer Cells.
    Article Snippet: .. Human renal cancer ACHN and A498 cells were obtained from ATCC (Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. .. Primary human renal proximal tubule epithelial cells (RPTECs) were obtained from ATCC (Manassas, VA, USA) and cultured in renal epithelial cell basal medium (ATCC® PCS-400–030TM) supplemented with a renal epithelial cell growth kit (ATCC® PCS-400–040TM).

    Modification:

    Article Title: Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancer.
    Article Snippet: .. Cultured human embryonic kidney (HEK293) (ATCC, Cat# CRL-1573; RRID:CVCL_0045), (Human, unknown) and HK-2 (ATCC, Cat# CRL-2190; RRID:CVCL_0302), (Human, Male) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich), 786-O cells (ATCC Cat# CRL-1932; RRID:CVCL_1051), (Human, Male) OS-RC-2 (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_1626) cells (Human, Male), and TUHR14TKB (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_5953) cells (Human, Male) in Roswell Park Memorial Institute (RPMI)1640 Medium (Sigma-Aldrich), A498 cells (ATCC Cat# CRL-44; RRID:CVCL_1056), (Human, Female) in Minimum Essential Medium (MEM, SigmaAldrich), Caki-1 (ATCC Cat# HTB-46; RRID:CVCL_0234), (Human, Male) and Caki-2 cells (ATCC Cat# HTB-47; RRID:CVCL_0235), (Human, Male) in McCoy’s 5A Medium (Sigma-Aldrich) all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich). .. Cultured human embryonic kidney (HEK293) (ATCC, Cat# CRL-1573; RRID:CVCL_0045), (Human, unknown) and HK-2 (ATCC, Cat# CRL-2190; RRID:CVCL_0302), (Human, Male) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich), 786-O cells (ATCC Cat# CRL-1932; RRID:CVCL_1051), (Human, Male) OS-RC-2 (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_1626) cells (Human, Male), and TUHR14TKB (RIKEN Cell Bank, Tsukuba, Japan, RRID:CVCL_5953) cells (Human, Male) in Roswell Park Memorial Institute (RPMI)1640 Medium (Sigma-Aldrich), A498 cells (ATCC Cat# CRL-44; RRID:CVCL_1056), (Human, Female) in Minimum Essential Medium (MEM, SigmaAldrich), Caki-1 (ATCC Cat# HTB-46; RRID:CVCL_0234), (Human, Male) and Caki-2 cells (ATCC Cat# HTB-47; RRID:CVCL_0235), (Human, Male) in McCoy’s 5A Medium (Sigma-Aldrich) all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich).

    Article Title: Synergistic and Selective Antiproliferative Effects of Cafestol and a Hyaluronic Acid-Epigallocatechin Gallate Conjugate in Human Renal Cancer Cells.
    Article Snippet: .. Human renal cancer ACHN and A498 cells were obtained from ATCC (Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. .. Primary human renal proximal tubule epithelial cells (RPTECs) were obtained from ATCC (Manassas, VA, USA) and cultured in renal epithelial cell basal medium (ATCC® PCS-400–030TM) supplemented with a renal epithelial cell growth kit (ATCC® PCS-400–040TM).



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    Image Search Results


    (A) Schematic depicting cancer cell reseeding on TK173-synthesized CDMs and further functional workup (Created in BioRender. Schell, C. (2026) https://BioRender.com/x5sqb3y ). (B) Volcano plot of RNA sequencing analysis depicting differentially expressed genes of A498 cancer cells after reseeding on TK173-synthesized CDMs (COL6-containing CDM or depleted CDM; red dots indicate significantly regulated genes with adjusted p<0.05 and log 2 fold change (FC) > |0.5|). (C) Gene ontology (GO) gene set enrichment analysis of GO-terms of the molecular function category. (D) Volcano plot analysis for filtered matrisome genes. (E) IF staining for Nucleus (blue), F-actin (white), FN (green) and COL6 (violet) of A498 cells reseeded on shCTRL- and shCOL6-CDMs. (F) Scanning electron microscopy of A498 cells interacting with CDMs (yellow dotted boxes highlight areas of zoom in). (G&H) Morphometric analysis of reseeded cells (cell area and perimeter; Violin plots of individual cells of N=3 independent experiments, Mann-WhitneyLULtest). (I) Quantification of confluence of A498 cells growing on shCTRL- and shCOL6-CDMs (N=4 independent experiments, unpaired t test). (J&K) Representative phase-contrast images with DNA staining (blue) of A498 cells after cultivation for 3 days. Dot plot depicting quantification of A498 cells per cm² grown on CDMs (N=4 independent experiments, unpaired t test). (L&M) Representative IF staining of ccRCC patient samples for DNA (blue), PAX8 (red), KI-67 (green) and COL6 (violet) was used to classify and mask (bottom image) for proliferating tumor cells (orange), non-proliferative tumor cells (grey), as well as COL6 (violet). Violin plot depicting quantification of the distance of non-proliferative and proliferating tumor cells to COL6 (Mann-WhitneyLULtest). (N) Heatmap depicting Spearman’s correlation analysis of indicated proliferative cell state marker genes with COL6 , SERPINE1 and SERPINE2 genes in the ccRCC TCGA and CPTAC cohorts. (O&P) Spatial transcriptomics analysis of ccRCCs for spatial co-occurrence of COL6 , SERPINE1 , SERPINE2 and proliferative cell state marker genes. (O) Exemplary spatial mapping of the co-occurrence score on one sample (PD47512) and spatial mapping for the COL6, SERPINE and proliferative gene scores. (P) Correlation matrix of the spatial correlation between the COL6, SERPINE and proliferative gene sets (N=5 patients, Spearman’s correlation). Bars indicate mean and S.E.M in dot plots or median and quartiles in violin plots; ∗ – p < 0.05, ∗∗ – p < 0.01 and ∗∗∗∗ – p < 0.0001.

    Journal: bioRxiv

    Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma

    doi: 10.64898/2026.03.19.712351

    Figure Lengend Snippet: (A) Schematic depicting cancer cell reseeding on TK173-synthesized CDMs and further functional workup (Created in BioRender. Schell, C. (2026) https://BioRender.com/x5sqb3y ). (B) Volcano plot of RNA sequencing analysis depicting differentially expressed genes of A498 cancer cells after reseeding on TK173-synthesized CDMs (COL6-containing CDM or depleted CDM; red dots indicate significantly regulated genes with adjusted p<0.05 and log 2 fold change (FC) > |0.5|). (C) Gene ontology (GO) gene set enrichment analysis of GO-terms of the molecular function category. (D) Volcano plot analysis for filtered matrisome genes. (E) IF staining for Nucleus (blue), F-actin (white), FN (green) and COL6 (violet) of A498 cells reseeded on shCTRL- and shCOL6-CDMs. (F) Scanning electron microscopy of A498 cells interacting with CDMs (yellow dotted boxes highlight areas of zoom in). (G&H) Morphometric analysis of reseeded cells (cell area and perimeter; Violin plots of individual cells of N=3 independent experiments, Mann-WhitneyLULtest). (I) Quantification of confluence of A498 cells growing on shCTRL- and shCOL6-CDMs (N=4 independent experiments, unpaired t test). (J&K) Representative phase-contrast images with DNA staining (blue) of A498 cells after cultivation for 3 days. Dot plot depicting quantification of A498 cells per cm² grown on CDMs (N=4 independent experiments, unpaired t test). (L&M) Representative IF staining of ccRCC patient samples for DNA (blue), PAX8 (red), KI-67 (green) and COL6 (violet) was used to classify and mask (bottom image) for proliferating tumor cells (orange), non-proliferative tumor cells (grey), as well as COL6 (violet). Violin plot depicting quantification of the distance of non-proliferative and proliferating tumor cells to COL6 (Mann-WhitneyLULtest). (N) Heatmap depicting Spearman’s correlation analysis of indicated proliferative cell state marker genes with COL6 , SERPINE1 and SERPINE2 genes in the ccRCC TCGA and CPTAC cohorts. (O&P) Spatial transcriptomics analysis of ccRCCs for spatial co-occurrence of COL6 , SERPINE1 , SERPINE2 and proliferative cell state marker genes. (O) Exemplary spatial mapping of the co-occurrence score on one sample (PD47512) and spatial mapping for the COL6, SERPINE and proliferative gene scores. (P) Correlation matrix of the spatial correlation between the COL6, SERPINE and proliferative gene sets (N=5 patients, Spearman’s correlation). Bars indicate mean and S.E.M in dot plots or median and quartiles in violin plots; ∗ – p < 0.05, ∗∗ – p < 0.01 and ∗∗∗∗ – p < 0.0001.

    Article Snippet: Human renal cancer cell lines A498 (HTB-44, ATCC,) and 786-O (CRL-1932, ATCC) as well as human renal fibroblasts TK173 (RRID: CVCL_C8FA) and human renal proximal tubule epithelial cells (RPTEC/TERT1, ATCC, CRL-4031) were used and obtained as recently described .

    Techniques: Synthesized, Functional Assay, RNA Sequencing, Staining, Electron Microscopy, Marker, Spatial Transcriptomics